图6:细胞质杂交种的特征是核苷酸歪斜的开关。

(a)在cytoDRIP峰值处的趋同转录模型。RNA-DNA杂化是在非模板链上高gc -歪斜区域的正义和反义转录的结果。示例核苷酸序列，适合观察到的歪斜模式显示。(b)细胞DRIP (n = 2911)和核DRIP (n = 65,541)区域AT含量(左)和GC含量(右)的小提琴图，细胞DRIP和核DRIP区域之间的双尾Mann Whitney U: p = 3.6e-8(左)，p = 3.5e-16(右)。中线，中间;方框限制，75和25百分位，胡须，最小值和最大值。(c)基因核DRIP区域(n = 56,433)周围的聚集图显示GC歪斜(左)和AT歪斜(右)。误差带代表平均值的95% CI。(d)基因siCtrl细胞drip区域周围的聚集图显示GC歪斜(左)和AT歪斜(右)。显示了282个峰值(n = 282)的均值; error bands represent 95% CI of the mean. (e) Aggregate plots around genic siCtrl cytoDRIP regions showing cytoDRIP–seq signal (left), nuclear DRIP–seq signal (middle) and nuclear RHR signal (right). Means of 282 peaks (n = 282) are shown; error bands represent 95% CI of the mean. (f) Violin plots of AT content (left) and GC content (right) for siSETX (n = 2629) and siCtrl (n = 282) cytoDRIP regions, two-tailed Mann Whitney U between cytoDRIP and nuclear DRIP regions: p = 0.003 (left), p = 0.003 (right). Centre line, median; box limits, 75 and 25 percentiles, whiskers, min and max values.